The MiSeq, which launched in the fall of 2011, is Illumina’s desktop or “personal sequencing system”. It is a smaller, faster and lower output sequencer, aimed primarily at the emerging sequencing-based diagnostics market. It is a self-contained system with onboard cluster generation, simplified reagent handling, and tight integration with Base Space, Illumina’s cloud-based data analysis platform. In November 2013 Illumina launched an FDA-cleared version of the instrument, called the MiSeqDx. Its MiSeq SBSv3 kit produces 2×300 bp read length and up to 15 Gb data in one run. We use this technology for denovo genome sequencing of smaller genomes, 16s Amplicon sequencing and sequencing of cancer panels.
