The Nucleome Informatics is the first Indian company to offer optical mapping on the Bionano Genomics Saphyr system. The Saphyr system uses a non-sequencing based optical mapping technology to analyse ultra-long strands of genomic DNA. This DNA is fluorescently labelled with the new Direct Labelling (DLS) technology and is imaged after individual DNA molecules are separated in thousands of channels on a nano-fluidic chip. Large structural variants, including insertions and deletions, translocations and inversions at a genomic scale, can be analysed with this system. A favourable method for researchers interested in, for example, plant trait development, domestication, or polyploidy. With high-quality software and improved computational resources, assembly, scaffolding, variant calling, and annotation is readily available for our customers. The Saphyr helps construct high precision genome maps, which can be used to build de novo genome assembly and superscaffolding of contig level genomes. The power of this combination can be found in an increase in the contiguity of the assembly to benefit both practically and economically.
